Methods for detecting or quantifying CTP and CTP synthase activity
AssigneesAssitance Publique—Hopitaux De Paris, Imagine Institut Des Maladies Genetiques Necker Enfants Malades, Universite Paris Descartes, Institut National De La Sante Et De La Recherche Medicale (Inserm), Centre National de la Recherche Scientifique (CNRS)
InventorsSylvia Sanquer, Anne-Claire Boschat, Sylvain Latour, Emmanuel Martin, Robert Barouki
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Abstract
The present invention relates to a method for detecting or quantifying CTP in a cell sample comprising at least two nucleotide triphosphates by cationic ion pairing chromatography coupled to mass spectrometry, to a method for detecting or quantifying CTP synthase activity based on the method for detecting or quantifying CTP, and to their use in methods for screening potential immunosuppressive or anti-cancer compounds and in methods for determining the appropriate dose of an immunosuppressive or anti-cancer compound inhibiting CTP synthase activity for a treated subject.
Claims19
A method for detecting or quantifying CTP in a sample comprising at least two distinct nucleotide triphosphate, said method comprising:
- a) separating the distinct nucleotide triphosphate by ion-pairing chromatography using a cationic ion pair reagent contained in a mobile phase having a pH comprised between 6 and 7, wherein the sample injected for the chromatography has a pH comprised between 1 and 4 and
- b) detecting or quantifying CTP by mass spectrometry.
Dependencies are inferred from the claim text.