Methods for producing ribosomally synthesized and posttranslationally modified peptides
AssigneeUniversity of Utah Research Foundation
InventorEric Schmidt
Life of this patent
Estimating the term…
Abstract
The disclosure provides recombinant cells and methods for producing a ribosomally synthesized and posttranslationally modified peptide (RiPP), as well as RiPP libraries and methods for producing RiPP libraries.
Claims6
A method for producing a ribosomally synthesized and posttranslationally modified peptide (RiPP), comprising combining a PatD or a TruD RiPP heterocyclase, a PatA or a TruA RiPP leader protease, a PatG or a TruG RiPP macrocyclase, RiPP heterocyclase substrate, and a sulfide source to form a mixture, under conditions sufficient to convert the RiPP heterocyclase substrate into a ribosomally synthesized and posttranslationally modified peptide;
- wherein the RiPP is produced in vivo in a bacterial cell and/or in vitro.
Dependencies are inferred from the claim text.