Oligosaccharide production
AssigneeGlycom A/S
InventorsEric Samain, Pauline Peltier-Pain, Katrine Bych, Ted Johanson, Elise Champion, Gyula Dekany
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Abstract
The invention relates to a genetically modified microorganism for making a recombinant oligosaccharide, preferably of 3-8 monosaccharide units, more preferably of 3-5 monosaccharide units, particularly a HMO, which comprises one or more genes encoding a sucrose utilization system, so the microorganism can use sucrose as a carbon and energy source. The one or more genes encoding a sucrose utilization system are preferably one or more genes encoding a heterologous PTS-dependent sucrose utilization transport system, such as the scr genes.
Claims8
A genetically modified microorganism capable of making a recombinant oligosaccharide, comprising:
- a recombinant gene encoding a glycosyl transferase which can transfer a glycosyl residue of an activated sugar nucleotide to a carbohydrate acceptor internalized by the microorganism,
- a biosynthetic pathway for making said activated sugar nucleotide from sucrose,
- one or more genes encoding a heterologous PTS-dependent sucrose utilization system so that said cell is capable to use sucrose as a carbon source for making said activated sugar nucleotide and as an energy source, and
- wherein said genetically modified microorganism is an E. coli cell of LacZ− genotype and comprises a galactosyl transferase capable of transferring a galactosyl residue of UDP-Gal to lacto-N-triose,
- said recombinant oligosaccharide is lacto-N-neotetraose (LNnT),
- said carbohydrate acceptor is lactose,
- said activated sugar nucleotide is UDP-GlcNAc,
- said glycosyl transferase is an N-acetyl-glucosaminyl transferase,
- said glycosyl residue is GlcNAc,
- said heterologous PTS-dependent sucrose utilization system comprises proteins encoded by scr genes scrY, scrA, scrB and scrR.
Dependencies are inferred from the claim text.