Recombinant microorganism for improved production of fine chemicals

A recombinant microorganism comprising:

• an introduced, increased, enhanced or altered activity and/or expression of a gcvA gene or a homolog or functional variant thereof,
• wherein the gcvA gene is operatively linked to a nucleic acid molecule comprising a sequence of SEQ ID NO:
• 56 or 57.

A method of producing pyruvate, succinate, aspartate, malate, lactate, valine, leucine and/or alanine comprising:

• culturing one or more recombinant microorganism under conditions that allow for the production of pyruvate, succinate, aspartate, malate, lactate, valine, leucine and/or alanine, wherein the microorganism comprises an introduced, increased, enhanced or altered activity and/or expression of a gcvA gene or a homolog or functional variant thereof;
• wherein the microorganism is cultured in a medium comprising between 0.5% and 30% (w/v) of a sugar.

A process for fermentative production of pyruvate, succinate, aspartate, malate, lactate, valine, leucine and/or alanine comprising the steps of

• I) growing a microorganism in a fermenter, wherein the microorganism comprises an introduced, increased, enhanced or altered activity and/or expression of a gcvA gene or a homolog or functional variant thereof, and
• II) recovering pyruvate, succinate, aspartate, malate, lactate, valine, leucine and/or alanine from the fermentation broth obtained in I).