US 10,731,187 B2 · Recombinant strain producing O-aminobenzoate and fermentative production of aniline from renewable resources via 2-aminobenzoic acid

Abstract

The invention provides a recombinant microbial host cell capable of converting a raw material comprising a fermentable carbon substrate to o-aminobenzoate biologically. The invention further provides a method for producing aniline, comprising the steps of: a) producing o-aminobenzoate by fermentation of a raw material comprising at least one fermentable carbon substrate using the recombinant microbial host cell of the capable of converting said raw material comprising at least one fermentable carbon substrate to o-aminobenzoate biologically, wherein said o-aminobenzoate comprises anthranilate anion, b) converting said o-aminobenzoate from said anthranilate anion to anthranilic acid by acid protonation, c) recovering said anthranilic acid by precipitation or by dissolving in an organic solvent, and d) converting said anthranilic acid to aniline by thermal decarboxylation in an organic solvent.

Claims45

Dependent claims

A recombinant microbial host cell capable of converting a raw material comprising a fermentable carbon substrate to o-aminobenzoate biologically,

wherein the microbial host cell is a Corynebacterium strain,
wherein said Corynebacterium strain is Corynebacterium glutamicum,
wherein said fermentable carbon substrate is selected from the group consisting of C-5 monosaccharides, C-6 monosaccharides, disaccharides, and tri-saccharides, and
wherein said Corynebacterium glutamicum has decreased expression of anthranilate phosphoribosyl transferase caused by reduced expression of trpD gene encoding anthranilate phosphoribosyl transferase, wherein said reduced expression is caused by a genetic modification of the trpD gene, and wherein said genetic modification is selected from the group consisting of SEQ ID NO:
2, SEQ ID NO:
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6, SEQ ID NO:
7, and SEQ ID NO:
8.

8 more elements

Dependencies are inferred from the claim text.

Recombinant strain producing O-aminobenzoate and fermentative production of aniline from renewable resources via 2-aminobenzoic acid

Life of this patent

Recombinant strain producing O-aminobenzoate and fermentative production of aniline from renewable resources via 2-aminobenzoic acid

Life of this patent

Abstract

Claims45