Composition and method for affecting the binding of antigen-binding polypeptides to antigens
AssigneeMiltenyi Biotech GmbH
InventorsStefan Dübel, Sarah-Jane Kellmann, Holger Thie
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Abstract
The present invention provides a composition comprising i) a polypeptide comprising calmodulin and two immunoglobulin superfamily domains, wherein said two immunoglobulin superfamily domains are linked via said calmodulin; ii) a calmodulin binding molecule; iii) ions binding to the Ca2+ binding site of calmodulin; wherein the binding of said calmodulin-binding molecule and of said ions to said Ca2+ binding site of calmodulin affects the binding of said polypeptide to an antigen to be bound by said polypeptide. The calmodulin may be a permutated calmodulin. A method for affecting the binding of a polypeptide for an antigen using said composition is also disclosed.
Claims5
A composition comprising
- i) a polypeptide comprising a single chain Fv (scFv) comprising a calmodulin and a variable region of a heavy chain of an immunoglobulin (VH) and a variable region of a light chain of the immunoglobulin (VL), wherein said VH and VL are linked via said calmodulin, wherein said calmodulin is a circularly permutated calmodulin,
- ii) a calmodulin binding peptide, wherein said calmodulin binding peptide is not M13 peptide,
- iii) Ca2+ ions,
- wherein the binding of said calmodulin-binding peptide and of said Ca2+ ions to Ca2+ binding site of said calmodulin affects the binding of said polypeptide to an antigen to be bound by said polypeptide,
- wherein said circularly permutated calmodulin has the sequence selected from the group consisting of sequences SEQ ID NO:
- 67 to SEQ ID NO:
- 123, and wherein said calmodulin binding peptide has the sequence selected from the group consisting of sequences SEQ ID NO:
- 1 to SEQ ID NO:
- 52 and SEQ ID NO:54 to SEQ ID NO:65.
Dependencies are inferred from the claim text.