Viable viruses with foreign tags
AssigneeThe United States of America as represented by the Secretary of Agriculture
InventorsKay S. Faaberg, Allyn Spear, Matthew A. Kappes, Kelly M. Lager
Life of this patent
Estimating the term…
Abstract
Multiple DIVA vaccines effective against porcine reproductive and respiratory syndrome virus (PRRSV) are disclosed. The DIVA vaccines may be negative DIVAs or positive DIVAs. The DIVA vaccines may be produced by modifying the nsp2 region of a modified live virus vaccine. The modification may be one or more deletions only (negative DIVAs) or a deletion with an insertion (positive DIVAs). The insertion may be of an epitope tag, such as a V5, S-Tag, or FLAG tag. Produced DIVA vaccines may be stable through multiple passes and thus may be effective for production and vaccination of animals.
Claims6
An immunogenic composition, comprising:
- an isolated polynucleotide sequence representing a modified live vaccine for porcine reproductive and respiratory syndrome virus having an nsp2 region;
- and
- a pharmaceutically acceptable adjuvant,
- wherein the amino acid sequence of the nsp2 region is one of a sequence of SEQ ID NO:
- 3;
- a sequence of SEQ ID NO:
- 13;
- a sequence of SEQ ID NO:
- 15;
- a sequence of SEQ ID NO:
- 17;
- a sequence having at least 99% homology to SEQ ID NO:
- 13;
- a sequence having at least 99% homology to SEQ ID NO:
- 15;
- and a sequence having at least 99% homology to SEQ ID NO:
- 17, and
- wherein the immunogenic composition is capable of inducing an immune response in a recipient.
An immunogenic composition, comprising:
- an isolated polynucleotide sequence representing a modified live vaccine for porcine reproductive and respiratory syndrome virus having an nsp2 region;
- and
- a pharmaceutically acceptable adjuvant,
- wherein the amino acid sequence of the nsp2 is one of a sequence of SEQ ID NO:
- 5;
- a sequence of SEQ ID NO:
- 19;
- a sequence of SEQ ID NO:
- 21;
- a sequence of SEQ ID NO:
- 23;
- a sequence having at least 99% homology to SEQ ID NO:
- 19;
- a sequence having at least 99% homology to SEQ ID NO:
- 21;
- and a sequence having at least 99% homology to SEQ ID NO:
- 23, and
- wherein the immunogenic composition is capable of inducing an immune response in a recipient.
A method for reducing the incidence of porcine reproductive and respiratory syndrome virus (PRRSV) in swine, comprising administering to a swine an immunogenic composition in an amount effective to generate an immune response in said swine to said PRRSV,
- wherein the immunogenic composition comprises an isolated polynucleotide sequence representing a modified live vaccine for porcine reproductive and respiratory syndrome virus having an nsp2 region,
- wherein the amino acid sequence of the nsp2 region is one of a sequence of SEQ ID NO:
- 3;
- a sequence of SEQ ID NO:
- 13;
- a sequence of SEQ ID NO:
- 15;
- a sequence of SEQ ID NO:
- 17;
- a sequence having at least 99% homology to SEQ ID NO:
- 13;
- a sequence having at least 99% homology to SEQ ID NO:
- 15;
- a sequence having at least 99% homology to SEQ ID NO:
- 17;
- a sequence of SEQ ID NO:
- 5;
- a sequence of SEQ ID NO:
- 19;
- a sequence of SEQ ID NO:
- 21;
- a sequence of SEQ ID NO:
- 23;
- a sequence having at least 99% homology to SEQ ID NO:
- 19;
- a sequence having at least 99% homology to SEQ ID NO:
- 21;
- and a sequence having at least 99% homology to SEQ ID NO:
- 23.
Dependencies are inferred from the claim text.