# US 10,725,031 B1 · Reflux device for detection of analytes in samples > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 10,725,031 B1 - Document ID: US10725031B1 - Status: Granted - Kind: B1 - Application number: 15/933,083 - Filed: 2018-03-22 - Granted: 2020-07-28 - Earliest claimed: 2017-04-26 - Assignees: ConquerAb Inc. - Applicants: Maria D. F. S. Barbosa - Inventors: Maria D. F. S. Barbosa - Web page: https://patentgrep.com/patent/US10725031B1 ## CPC - G01N 33/54388: {based on lateral flow} - B01L 3/5023: {with a sample being transported to, and subsequently stored in an absorbent for analysis} - G01N 33/54393: {Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding} - B01L 2200/0647: Handling flowable solids, e.g. microscopic beads, cells, particles - B01L 2200/10: Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept - B01L 2200/16: Reagents, handling or storing thereof - B01L 2300/047: Additional chamber, reservoir - B01L 2300/0681: Filter - B01L 2300/069: Absorbents; Gels to retain a fluid - B01L 2300/0825: Test strips - B01L 2300/0867: Multiple inlets and one sample wells, e.g. mixing, dilution - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE ## Abstract Devices for detection of analytes in samples of various complexities are provided. The devices allow reflux of liquid into a sample-receiving member, providing for suitable analyte dilution and preventing leakage of cells or particulate material into the signal generation member. In one aspect, the devices are used to test analytes in biological samples such as blood, serum, plasma or other body fluids. In another aspect, the devices are used to test samples from tissues, such as organ tissues, tumor samples, or other body parts. In a further aspect, the devices are used to test microbial samples, food samples, or any other sample from which the analyte of interest can be captured into the reflux device. ## Claims ### Claim 1 A device comprising: a. A sample-receiving port adjacent to a sample-collecting pad which is configured to allow a sample to be placed on the sample-collecting pad at a sample-collecting point; b. A detection zone which produces a signal in response to a sample analyte from the sample interacting with the detection zone, wherein the device is configured to cause the sample analyte to travel from the sample-collecting spot to the detection zone in a downstream direction; c. A first liquid reservoir placed in the downstream direction from the sample-receiving port and at a level above the sample-collecting pad, wherein said first liquid reservoir has a first liquid in a predetermined amount and the device is configured such that when the first liquid is released, the first liquid travels in an upstream direction, opposite to the downstream direction, to the sample-collecting point and then travels in the downstream direction after reaching the sample-collecting point; and d. A second liquid reservoir placed in the downstream direction from the first reservoir, wherein said second liquid reservoir has a second liquid and the device is configured such that when the second liquid is released, the second liquid travels in the downstream direction. ### Claim 2 (depends on 1) The device of claim 1, wherein the sample-receiving pad can retain one or more particulates selected from the group consisting of blood cells, microorganisms, tissue debris, solids in tumor homogenates, solids in tissue homogenates, particulate matter in testing samples. ### Claim 3 (depends on 1) The device of claim 1, further comprising a filter positioned in the downstream direction from the sample-receiving pad. ### Claim 4 (depends on 1) The device of claim 1, further comprising a connecting pad that allows the sample to flow towards one or more reagents immobilized on the detection zone, and wherein binding of the sample analyte with the one or more reagents results in the signal. ### Claim 5 (depends on 4) The device of claim 4, wherein the device is configured to quantify the signal generated upon binding of the sample analyte to the one or more immobilized reagents. ### Claim 6 (depends on 1) The device of claim 1, further comprising an attached lancet. ### Claim 7 (depends on 1) A system comprising the device of claim 1, and further comprising encapsulated blood drops or blood samples of measured volumes, wherein said measured volumes are provided on the device itself or on an accompanying tool or set or instructions. ### Claim 8 A device comprising: a. A sample-receiving port adjacent to a sample-collecting pad which is configured to allow a sample to be placed on the sample-collecting pad at a sample-collecting point; b. A detection zone which produces a signal in response to a sample analyte from the sample interacting with the detection zone, wherein the device is configured to cause the sample analyte to travel from the sample-collecting spot to the detection zone in a downstream direction; and c. A liquid reservoir placed in the downstream direction from the sample-receiving port and at a level above the sample-collecting pad, wherein said liquid reservoir has a liquid in a predetermined amount and the device is configured such that when the liquid is released, the liquid travels in an upstream direction, opposite to the downstream direction, to the sample-collecting point and then travels in the downstream direction after reaching the sample-collecting point. ### Claim 9 (depends on 8) The device of claim 8, wherein the sample-receiving pad can retain one or more particulates selected from the group consisting of blood cells, microorganisms, tissue debris, solids in tumor homogenates, solids in tissue homogenates, particulate matter in testing samples. ### Claim 10 (depends on 8) The device of claim 8, further comprising a filter positioned in the downstream direction from the sample-receiving pad. ### Claim 11 (depends on 8) The device of claim 8, further comprising a connecting pad that allows the sample to flow towards one or more reagents immobilized on the detection zone, and wherein binding of the sample analyte with the one or more reagents results in the signal. ### Claim 12 (depends on 11) The device of claim 11, wherein the device is configured to quantify the signal generated upon binding of the sample analyte to the one or more immobilized reagents. ### Claim 13 (depends on 8) The device of claim 8, further comprising an attached lancet. ### Claim 14 (depends on 8) A system comprising the device of claim 8, and further comprising encapsulated blood drops or blood samples of measured volumes, wherein said measured volumes are provided on the device itself or on an accompanying tool or set or instructions.