Methods and systems for analysis of chromatin
Assignee10x Genomics, Inc.
InventorsJason Bell, Geoffrey McDermott, Francesca Meschi, Michael Schnall-Levin, Xinying Zheng
Life of this patent
Estimating the term…
Abstract
The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from a single cell. Such polynucleotide processing may be useful for a variety of applications. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins) and chromatin (e.g., accessible chromatin).
Claims30
A method of analyzing chromatin, comprising:
- (a) providing a mixture comprising (i) a cell or nucleus comprising (1) chromatin comprising a template deoxyribonucleic acid (DNA) and (2) a protein, and (ii) a plurality of nucleic acid barcode molecules;
- (b) contacting said cell or nucleus with a labelling agent comprising a reporter oligonucleotide such that said labelling agent couples to said protein;
- (c) contacting said chromatin with a plurality of transposase complexes, thereby generating a plurality of template DNA fragments;
- (d) generating a first barcoded nucleic acid molecule comprising (i) a sequence of a template DNA fragment of said plurality of template DNA fragments and (ii) a sequence of a first nucleic acid barcode molecule of said plurality of nucleic acid barcode molecules;
- and
- (e) generating a second barcoded nucleic acid molecule comprising (i) a sequence of said reporter oligonucleotide and (ii) a sequence of a second nucleic acid barcode molecule of said plurality of nucleic acid barcode molecules.
Dependencies are inferred from the claim text.