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US 10,724,062 B2Granted
Biosynthetic production of steviol glycosides and processes therefore
AssigneeConagen Inc.
InventorsGuohong Mao, Jacob Edward Vick, Michael Batten, David Byun, Yang Luo, Yilin Wu, Beihua Zhang, Xiaodan Yu
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Abstract
The present invention relates to the production of steviol glycoside rebaudiosides D4, WB1 and WB2 and the production of rebaudioside M from Reb D4.
Claims15
A method of preparing a steviol glycoside composition, the method comprising:
- preparing a reaction mixture comprising:
- (a) rebaudioside W having the structure:
- and
- (b) a beta-glucosidase;
- and
- incubating the reaction mixture for a sufficient time to produce a steviol glycoside composition comprising rebaudioside WB1, rebaudioside WB2, or a combination thereof, wherein rebaudioside WB1 has the structure:
- and rebaudioside WB2 has the structure:
4 more elements
Rebaudioside WB2 having the structure:
Rebaudioside D4 having the structure:
Dependencies are inferred from the claim text.