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US 10,724,010 B2Granted
Recombinantly engineered cells expressing chlorite dismutase and methods for using same in cell culture
AssigneeThe Regents of the Uniiversity of California
InventorJohn D. Coates
Life of this patent
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Abstract
The present disclosure relates to recombinantly engineered cells that contain a chlorite dismutase polypeptide and methods for culturing such cells in a culture medium containing chlorite in an amount sufficient to reduce the growth rate or kill contaminating microorganisms without killing the recombinantly engineered cells. Also provided are methods for the production of a fermentation product using the recombinantly engineered cells.
Claims20
A method of culturing a cell, comprising:
- a) culturing a cell recombinantly engineered to express a functional chlorite dismutase in a culture medium under conditions whereby the chlorite dismutase is expressed in said cell, wherein said culture medium comprises one or more contaminating microorganisms, wherein the chlorite dismutase is expressed in the cell from a chlorite dismutase gene (cld) heterologous to the cell;
- and
- b) treating said culture medium with chlorite in an amount sufficient to kill said one or more contaminating microorganisms without killing said cell, wherein the chlorite dismutase protects the cell from toxic effects of the chlorite.
Dependencies are inferred from the claim text.