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US 10,717,998 B2Granted
Recombinant microorganism for improved production of fine chemicals
OwnerJennifer Jaitzig, Mukesh Kumar +3inventors
InventorsJennifer Jaitzig, Mukesh Kumar, Shakir Ratani, Matthew Blankschien, Qingzhao Wang
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Abstract
The present invention relates to a recombinant microorganism, to a method for producing alanine and to the use of the recombinant microorganism for the fermentative production of alanine.
Claims25
A recombinant microorganism comprising:
- (1) an introduced, increased or enhanced activity and/or expression of a gcvTHP operon, wherein the change in activity is in comparison to a respective reference microorganism without introduced, increased or enhanced activity of a gcvTHP operon, and wherein the gcvTHP operon is selected from the group consisting of:
- (i) a nucleic acid molecule comprising SEQ ID NO:
- 51,
- (ii) a nucleic acid molecule having at least 80% identity to SEQ ID NO:
- 51,
- (iii) a nucleic acid molecule encoding each of the polypeptides SEQ ID NO:
- 46, 48 and 50, and
- (iv) a nucleic acid molecule encoding a polypeptide having at least 60% homology to the polypeptide of SEQ ID NO:
- 46 and a polypeptide having at least 60% homology to the polypeptide of SEQ ID NO:
- 48 and a polypeptide at least 60% homology to the polypeptide of SEQ ID NO:
- 50 and;
- (2) a reduced, repressed or deleted activity and/or expression of an adhE gene, wherein the change in activity is in comparison to a respective reference microorganism without reduced, repressed or deleted activity and/or expression of adhE.
9 more elements
A method for producing a recombinant microorganism with enhanced pyruvate, succinate, aspartate, malate, lactate, valine, leucine and/or alanine yield and/or productivity, which comprises the following steps:
- (I) introducing, increasing or enhancing the activity and/or expression of a gcvTHP operon in a microorganism, wherein the change in activity is in comparison to a respective reference microorganism without introduced, increased or enhanced activity of a gcvTHP operon, wherein the gcvTHP operon is selected from the group consisting of:
- (i) a nucleic acid molecule comprising SEQ ID NO:
- 51,
- (ii) a nucleic acid molecule having at least 80% identity to SEQ ID NO:
- 51,
- (iii) a nucleic acid molecule encoding each of the polypeptides SEQ ID NO:
- 46, 48 and 50, and
- (iv) a nucleic acid molecule encoding a polypeptide having at least 60% homology to the polypeptide of SEQ ID NO:
- 46 and a polypeptide having at least 60% homology to the polypeptide of SEQ ID NO:
- 48 and a polypeptide at least 60% homology to the polypeptide of SEQ ID NO:
- 50;
- and
- (II) reducing, repressing or deleting activity and/or expression of an adhE gene, wherein the change in activity is in comparison to a respective reference microorganism without reduced, repressed or deleted activity and/or expression of adhE;
- and
- (III) generating a recombinant microorganism with enhanced pyruvate, succinate, aspartate, malate, lactate, valine, leucine and/or alanine yield and/or productivity compared to a respective reference microorganism without reduced, repressed or deleted activity and/or expression of the gcvTHP operon and without reduced, repressed or deleted activity and/or expression of adhE.
12 more elements
Dependencies are inferred from the claim text.