Recombinant yeast cells producing polylactic acid and uses thereof
AssigneesCarbios, Institut National De Recherche Pour L'agriculture, A'alimentation Et L'environnement, Institut National Des Sciences Appliquees, Centre National De La Recherche Scientifique
InventorsSimon Dusseaux, Sophie Lajus, Vinciane Borsenberger, Jonathan Verbeke, Florence Bordes, Alain Marty, Jean-Marc Nicaud, Athanasios Beopoulos
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Abstract
The present invention relates to a recombinant yeast cell comprising a gene encoding a protein exhibiting lactyl-CoA synthase activity and a gene encoding a protein exhibiting lactyl-CoA polymerase activity, said recombinant cell having the ability of producing polylactic acid (PLA), and the uses thereof.
Claims20
A recombinant yeast cell suitable for producing polylactic acid (PLA), said recombinant cell comprising and expressing
- a gene encoding a lactyl-CoA synthase wherein the lactyl-CoA synthase is selected from the group consisting of a CoA transferase, a propionyl-CoA transferase (Pctp) and a CoA ligase, and
- a gene encoding a polyhydroxyalkanoate (PHA) synthase (PhaCp), wherein the recombinant yeast cell has been engineered for expressing the lactyl-CoA synthase in the cytosol and for expressing the PHA synthase in the peroxisome of said recombinant yeast cell.
Dependencies are inferred from the claim text.