Alkaline protease variant
AssigneeKao Corporation
InventorsKozo Yamada, Tsuyoshi Sato
Life of this patent
Estimating the term…
Abstract
Provided is an alkaline protease mutant with improved stability to a chelating agent. An alkaline protease mutant, in which an amino acid residue at a position corresponding to position 294 of the amino acid sequence as shown in SEQ ID NO: 2 is substituted in the amino acid sequence as shown in SEQ ID NO: 2, or an amino acid sequence having an identity of at least 95% thereto.
Claims20
An alkaline protease mutant, the amino acid sequence of the mutant consisting of
- (a) the amino acid sequence of SEQ ID NO:2, or
- (b) an amino acid sequence having at least 97% homology to SEQ ID NO:2,
- but in which, in the amino acid sequence of (a) and (b), the amino acid residue at position 294 in the amino acid sequence shown in SEQ ID NO:2, or at a position corresponding thereto, is a threonine.
A polynucleotide encoding an alkaline protease mutant, the amino acid sequence of the mutant consisting of
- (a) the amino acid sequence of SEQ ID NO:2, or
- (b) an amino acid sequence having at least 97% homology to SEQ ID NO:2,
- but in which, in the amino acid sequence of (a) and (b), the amino acid residue at position 294 in the amino acid sequence shown in SEQ ID NO:2, or at a position corresponding thereto, is a threonine.
A method for producing an alkaline protease mutant, comprising substituting the amino acid residue at a position corresponding to position 294 of the amino acid sequence as shown in SEQ ID NO:
- 2 with threonine, wherein the substituting is made to the amino acid sequence shown in SEQ ID NO:
- 2, or to an amino acid sequence having an identity of at least 97% to SEQ ID NO:
- 2.
A method for improving stability of alkaline protease to a chelating agent, comprising substituting the amino acid residue at a position corresponding to position 294 of the amino acid sequence as shown in SEQ ID NO:
- 2 with threonine, wherein the substituting is made to the amino acid sequence shown in SEQ ID NO:
- 2, or to an amino acid sequence having an identity of at least 97% to SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.