Engineered primate cystine/cysteine degrading enzymes as antineogenic agents
AssigneeBoard of Regents, The University of Texas System
InventorsGeorge Georgiou, Everett Stone
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Abstract
Methods and compositions related to the engineering of a protein with L-cyst(e)ine degrading enzyme activity are described. For example, in certain aspects there may be disclosed a modified cystathionine-γ-lyase comprising one or more amino acid substitutions and capable of degrading L-cyst(e)ine. Furthermore, certain aspects of the invention provide compositions and methods for the treatment of cancer with L-cyst(e)ine using the disclosed proteins or nucleic acids.
Claims11
A polypeptide comprising a variant of a native primate cystathionine-gamma-lyase (CGL) enzyme, wherein the variant CGL has cysteine/cystine degrading activity, comprises a sequence at least 95% identical to SEQ ID NO:
- 1, and comprises E59T and E339V substitutions relative to SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.