Methods of producing omega-hydroxylated fatty acid derivatives
AssigneeGenomatica, Inc.
InventorsAndreas W. Schirmer, Haibo Wang, Stephen B. Del Cardayre, Zhihao Hu, Louis G. Hom, Baolong Zhu, Cindy Chang, Emanuela E. Popova
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Abstract
The disclosure relates to omega-hydroxylated fatty acid derivatives and methods of producing them. Herein, the disclosure encompasses a novel and environmentally friendly production method that provides omega-hydroxylated fatty acid derivatives at high purity and yield. Further encompassed are recombinant microorganisms that produce omega-hydroxylated fatty acid derivatives through selective fermentation.
Claims17
A recombinant microorganism engineered to express a CYP153A-reductase hybrid fusion polypeptide variant comprising a CYP153A ω-hydroxylase domain and a P450RhF reductase domain;
- wherein the hybrid fusion polypeptide variant has at least 91% sequence identity to SEQ ID NO:
- 6 and has one or more mutations selected from the group consisting of V141I, V141T, V141Q, V141G, V141M, V141L, R27L, R82D, R178N, A231T, N309R, N407A, V415R, T516V, P666A, P666D and A796V.
Dependencies are inferred from the claim text.