Plant regulatory elements and methods of use thereof
AssigneesPioneer Hi-Bred International, Inc., E. I. Du Pont de Nemours and Company
InventorsScott Diehn, Albert L. Lu, Michelle Van Allen
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Abstract
The present disclosure provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for regulatory elements from Eupatorium Vein Clearing Virus. A method for expressing a heterologous nucleotide sequence in a plant using the regulatory element sequences disclosed herein is provided. The method comprises transforming a plant or plant cell with a nucleotide sequence operably linked to one of the regulatory elements of the present disclosure.
Claims18
A recombinant nucleic acid molecule comprising a regulatory element operably linked to a heterologous polynucleotide, wherein the regulatory element comprises a polynucleotide selected from the group consisting of:
- (a) the polynucleotide of SEQ ID NO:
- 1, and
- (b) a fragment of at least 283 nucleotides of SEQ ID NO:
- 1;
- and
- and wherein the regulatory element has regulatory activity in a plant on the heterologous polynucleotide.
A DNA construct comprising
- (a) a regulatory element selected from the group consisting of:
- (i) the polynucleotide of SEQ ID NO:
- 1, and
- (ii) a fragment of at least 283 nucleotides of SEQ ID NO:
- 1;
- and
- (b) a heterologous transcribable polynucleotide molecule operably linked to the regulatory element.
Dependencies are inferred from the claim text.