Capsules containing mammalian cells
AssigneeCapsum
InventorsJerome Bibette, Nicolas Atrux-Tallau, Hugo Domejean, Anette Funfak, Nicolas Bremond, Pierre Nassoy, Kevin Alessandri
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Abstract
A capsule containing at least one mammalian cell, includes a liquid core, and at least one external envelope totally encapsulating the liquid core at its periphery, the external envelope including at least one gelled polyelectrolyte and/or a stiffened biopolymer and being able to retain the liquid core when the capsule is immersed in a gas. The present invention further relates to the method for preparing such a capsule, to a method for screening cosmetic active ingredients as well as a culture method using such capsules.
Claims13
A microcapsule comprising:
- a liquid core;
- a stiff intermediate envelope comprising at least one biopolymer;
- and
- at least one external envelope totally encapsulating the liquid core at its periphery,
- said intermediate envelope being located between the liquid core and the external envelope,
- said external envelope being able to retain the liquid core when the microcapsule is immersed in a gas and comprising at least one gelled polyelectrolyte and/or one stiffened biopolymer,
- said microcapsule further comprising at least one eukaryotic mammalian cell, wherein the liquid core comprises at least one keratinocyte and the intermediate envelope comprises at least one fibroblast;
- and
- said stiff intermediate envelope having an elastic modulus that is non-zero.
Dependencies are inferred from the claim text.