System and method for automatically analyzing phenotypical responses of cells

A system to analyze a phenotypical response of cells to a treatment, comprising:

• a high-content imaging system, wherein the high-content imaging system includes an image capture device;
• and
• a response analysis system that includes computer-executable code stored on one or more non-transitory storage devices that, when executed, causes one or more processors to:
• receive images of a plurality of reference cell carriers and treatment information associated with the plurality of reference cell carriers, identify parameters of cells in the plurality of reference cell carriers from the images based on the treatment information, the parameters distinguishing those cells in the plurality of reference cell carriers to which the treatment has been applied from other cells in the plurality of reference cell carriers, and train a model using the identified parameters,
• receive from the image capture device a plurality of images of cell carriers to be evaluated, and
• apply the trained model to the plurality of images of the cell carriers to be evaluated to indicate a response level of cells in each of the cell carriers to be evaluated.