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US 10,705,099 B2Granted
Methods and probes for determining the concentration of copper
AssigneeIgea Research Corporation
InventorNicola Antonio Colabufo
Life of this patent
Estimating the term…
Abstract
The present invention relates to new probes and methods for determining the concentration of copper. In particular the present invention refers to a method for determining the concentration of “free” copper in a serum or blood sample with a high degree of sensitivity and accuracy.
Claims13
A method for determining free copper in a liquid sample, comprising
- mixing one or more Cu2+ chelating probes with said sample;
- and
- measuring a color change in the sample to determine the concentration of free copper;
- wherein said probe comprises:
- 4-(Diethylamino)-2-hydroxy-benzoyl(fluorescein)hydrazine;
- 2,3-Dihydro-5,7-dihydroxy-2-(3-hydroxy-4-methoxyphenyl)chromen-4-(fluorescein)hydrazone;
- 5-(2-Hydroxybenzylideneamino)quinolin-8-ol;
- Aldehyde,2-hydroxyphenyl-, [2,3-dihydro-5,7-dihydroxy-2-(3-hydroxy-4-methoxyphenyl)chromen-4-ylidene]hydrazine;
- 5-(4-(Diethylamino)-2-hydroxybenzylideneamino)quinolin-8-ol;
- Aldehyde,2-hydroxyphenyl-[(5-hydroxy-3-methyl-1-phenyl-1H-pyrazol-4yl)-phenyl)-methylidene] hydrazine;
- or
- combinations thereof.
9 more elements
A method of diagnosing a predisposition to Alzheimer's disease in a patient suffering from mild cognitive impairment (MCI) comprising:
- mixing one or more Cu2+ chelating probes with an extracted sample from the patient;
- and
- measuring a color change in the sample,
- wherein the one or more probes comprises
- 4-(Diethylamino)-2-hydroxy-benzoyl(fluorescein)hydrazine;
- 2,3-Dihydro-5,7-dihydroxy-2-(3-hydroxy-4-methoxyphenyl)chromen-4-(fluorescein)hydrazone;
- 5-(2-Hydroxybenzylideneamino)quinolin-8-ol;
- Aldehyde,2-hydroxyphenyl-,[2,3-dihydro-5,7-dihydroxy-2-(3-hydroxy-4-methoxyphenyl)chromen-4-ylidene]hydrazine;
- 5-(4-(Diethylamino)-2-hydroxybenzylideneamino)quinolin-8-ol;
- Aldehyde,2-hydroxyphenyl-[(5-hydroxy-3-methyl-1-phenyl-1H-pyrazol-4yl)-phenyl)-methylidene]hydrazine;
- or any combination thereof;
- and
- wherein a measured color change in said sample exceeding a threshold value (cut-off) is indicative of the predisposition to Alzheimer's disease in the patient.
10 more elements
Dependencies are inferred from the claim text.