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US 10,705,085 B2Granted
Tethered lipoplex nanoparticle biochips and methods of use
AssigneeOhio State Innovation Foundation
InventorsLy James Lee, Kwang Joo Kwak, Bo Yu, Yun Wu, Andrew Lee
Life of this patent
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Abstract
Disclosed are compositions and methods for the use of lipoplex nanoparticle chips and arrays in the detection of/diagnosis of a disease or condition.
Claims13
A method for detecting the presence or absence of a selected disease or condition in a subject from a sample of tissue or body fluid of the subject, comprising the steps of:
- providing a lipoplex nanoparticle, comprising a liposome having at least one molecular probe incorporated therein and at least one modification of the surface of the liposome to facilitate the surface for binding to a target feature that is characteristic of the presence or absence of the selected disease or condition, the lipoplex nanoparticle being tethered to a substrate;
- contacting the lipoplex nanoparticle with an amount of the sample from the subject;
- and
- detecting excitation of a label of the at least one molecular probe, the excitation occurring from the capture and incorporation into the lipoplex nanoparticle of the target feature;
- wherein the target feature is selected from the group consisting of:
- a cell-secreted extracellular vesicle and a virus.
3 more elements
A method for detecting the presence or absence of a selected disease or condition in a subject from a sample of tissue or body fluid of the subject, comprising the steps of:
- providing a biochip comprising a substrate on which at least one array of lipoplex nanoparticles are tethered;
- each array comprising a plurality of the lipoplex nanoparticles that comprise a liposome having at least one molecular probe incorporated therein and at least one modification of the surface of the liposome to facilitate the surface for binding to a target feature that is characteristic of the presence or absence of the selected disease or condition;
- contacting the biochip with an amount of the sample from the subject;
- and
- detecting excitation of the molecular probe of at least one of the arrays, the excitation occurring from the capture and incorporation into the lipoplex nanoparticle of the target feature;
- wherein the target feature is selected from the group consisting of:
- a cell-secreted extracellular vesicle and a virus.
4 more elements
Dependencies are inferred from the claim text.