Bioparticle characterization and identification using interface thermal resistance measurement during bioparticle adhesion and detachment

A bio-sensing device suitable for detection and/or characterization of target bioparticles, the bio-sensing device comprising:

• a heating element for heating using a power;
• a sample holder comprising a substrate having a surface comprising a plurality of binding sites to which target bioparticles can bind, the sample holder further being adapted for exposing the substrate at one side to the heating element;
• a first temperature sensing element for sensing a temperature at the side where the substrate can be exposed to the heating element and a second temperature sensing element for sensing a temperature at the side opposite thereto with respect to the substrate;
• and
• a processor programmed for determining, for at least one temperature, data representative of thermal resistance data based on temperature values obtained with the first temperature sensing element and the second temperature sensing element and the power for the heating element,
• wherein the processor is programmed for deriving, for at least the one temperature, a bioparticle retention time and/or detachment rate from the data representative of the thermal resistance data.