Method for preparing magnetic microsphere for separation of biological protein and use thereof

A method for preparing a magnetic microsphere for separation of biological protein, characterized in that it comprises:

• a. preparing an emulsion;
• b. dispersing a magnetic microsphere matrix with magnetism into the emulsion to generate a dispersion system;
• and
• c. subjecting the dispersion to a polymerization reaction;
• wherein the magnetic microsphere matrix is a magnetic particle modified with an active group on surface thereof;
• further wherein the active group is at least one selected from the group consisting of:
• a carboxyl group and a sulfonic acid group;
• and
• wherein the emulsion comprises the following components:
• an acrylic monoester compound selected from the group consisting of methyl methacrylate, ethyl methacrylate, hydroxyethyl methacrylate, n-propyl methacrylate, hydroxypropyl methacrylate, n-butyl methacrylate, and hydroxybutyl methacrylate;
• an acrylic diol ester compound selected from the group consisting of glycol dimethacrylate, 1,3-propanediol dimethacrylate, 1,3-butanediol dimethacrylate, 1,4-butanediol dimethacrylate, neopentyl glycol dimethacrylate and 1,6-hexanediol dimethacrylate;
• an anionic surfactant selected from the group consisting of sodium dodecyl sulfate, sodium dodecyl sulfonate, sodium hexadecyl sulfonate, and sodium n-decyl sulfate;
• water;
• and a water-soluble initiator selected from the group consisting of sodium persulfate, potassium persulfate, and ammonium persulfate.