Anti-HLA monoclonal chimeric immunoglobulin, method and kit implementing such a monoclonal chimeric immunoglobulin

A process for producing a calibration curve, the process comprising:

• preparing a plurality of solutions (Sn) of a monoclonal chimeric immunoglobulin, each solution (Sn) having a defined concentration value (Cn) of said monoclonal chimeric immunoglobulin, and then
• placing each solution (Sn) in contact with the same defined quantity of at least one immobilized HLA class II antigen, and
• producing the calibration curve, in which each defined concentration value (Cn) is associated with a measured value (Vn) of a parameter, said measured value (Vn) representing a quantity (Qn) of the monoclonal chimeric immunoglobulin linked to the defined quantity of each immobilized HLA class II antigen,
• forming from pairs (Cn, Vn) of defined concentration (Cn) and measured value (Vn), the calibration curve showing the variation of the measured value (Vn) as a function of the defined concentration (Cn) of monoclonal chimeric immunoglobulin of each solution (Sn) of monoclonal chimeric immunoglobulin,
• wherein the monoclonal chimeric immunoglobulin consists of:
• two polypeptide heavy chains (H) of molecular weight from 40 kDa to 60 kDa, and
• two polypeptide light chains (L) of molecular weight from 20 kDa to 30 kDa, wherein:
• each heavy chain (H) comprises:
• a heavy chain variable region (VH) of a monoclonal antibody selected from the group consisting of monoclonal antibodies specific to monomorphic epitopes of HLA class II, and
• a heavy chain constant region (CH) of a human immunoglobulin selected from the group consisting of IgAs, IgGs and IgMs,
• and wherein:
• each light chain (L) comprises:
• a light chain variable region (VL) of a monoclonal antibody selected from the group consisting of monoclonal antibodies specific to monomorphic epitopes of HLA class II antigens, and
• a light chain constant region (CO of a human immunoglobulin that is a kappa chain
• and wherein the two polypeptide heavy chains (H) are identical and the two polypeptide light chains (L) are identical, where
• light chains (L) are of sequence SEQ ID NO:
• 5, and
• heavy chains (H) are identical and selected from the group consisting of sequence SEQ ID NO:
• 6, sequence SEQ ID NO:
• 7 and sequence SEQ ID NO:
• 8.