A method of preparing and analyzing one or more slides according to an immuno-fluorescence technique with an automated machine, comprising the steps of:
- providing the automated machine comprising:
- a loading station of one or more containers for holding test liquids and product samples;
- a positioning station of one or more slides comprising a support base provided with a plurality of seats each configured to hold one of the one or more slides, each seat having a base and a containment frame configured to hold one slide, the positioning station being removable from the machine;
- a containment station of one or more first slide covers;
- an analysis station comprising a microscope for acquisition of one or more digital images at one or more magnifications;
- one or more second slide covers being sized to cover the one slide arranged in one of the seats,
- wherein each of the one or more second slide covers has a top surface, provided with a gripping handle, and lateral walls extending orthogonally from the top surface in a direction opposite to the gripping handle, each of the one or more second slide covers being rectangular in shape and being sized to enclose within, in a volume defined by the top surface and the lateral walls, the containment frame and the one slide disposed within the containment frame, each seat receiving silica gel and the one slide on the silica gel, so as to cause a combined effect of the one or more second slide covers with the silica gel that reduces deterioration time of substrates of the slides;
- a container holding the one or more second slide covers, the container being removable;
- and
- a loading system, mobile along perpendicular directions inside an entire area defined by the machine, the loading system removing one of the second slide covers from one of the slides positioned in one of the seats after pre-dilution of the product samples has been completed, and arranging the removed second slide covers inside the container,
- wherein the loading system comprises,
- a gripping device, configured to collect the one of the second slide covers and to deposit the one of the second slide covers in the container, the gripping device being further configured to carry the one of the one or more slides coupled with one of the first slide covers from the one of the seats to the microscope;
- a suction device, configured to collect the one of the first slide covers from the containment station and to deposit the one of the first slide covers on the one of the one or more slides positioned in the one of the seats, and
- one or more aspirating/application needles of a liquid, the gripping device, the suction device, and the one or more aspirating/application needles being jointly mobile along the perpendicular directions,
- wherein the loading system is further configured to move through the loading, positioning, containment, and analysis stations to prepare the one or more slides according to a pre-defined protocol while leaving the slides on the support base, and, after said preparation of the one or more slides, move the one or more slides to the analysis station for the acquisition of the one or more digital images, and
- wherein the loading system is mobile along the perpendicular directions by sliding along perpendicular sliding tracks disposed in an upper portion of the machine;
- arranging, in the loading station of the automated machine, the one or more containers for holding the test liquids and the product samples;
- arranging, in the positioning station of the automated machine, the one or more slides each lodged in one or more of the seats for holding the one of the one or more slides, wherein arranging the one or more slides comprises inserting the silica gel in the seat and disposing the slide on the silica gel;
- covering each slide with one of the second slide covers;
- arranging, in the containment station, the one or more first slide covers;
- causing the loading system to move through the loading, positioning, and containment stations to prepare one or more of the slides in accordance with the pre-defined protocol and arrange the one or more slides at the analysis station comprising a-the microscope for the acquisition of the digital images at the one or more magnifications, so as to make a subsequent acquisition of one or more digital images,
- wherein preparing the one or more slides comprises:
- removing the respective second slide covers, once pre-dilution of the product samples has been completed, and subsequently arranging the respective second slide covers inside the container;
- preparing the one or more slides in accordance with the pre-defined protocol, leaving the one or more slides in the respective seats, and
- after preparing the one or more slides, applying the one or more first slide covers on the one or more slides, and moving the one or more slides to the analysis station for the subsequent acquisition of the one or more digital images.