Marker associated with everbearing properties in plant of genus Fragaria and use thereof
AssigneeToyota Jidosha Kabushiki Kaisha
InventorsHiroaki Koishihara, Hiroyuki Enoki, Masayoshi Muramatsu, Satoru Nishimura, Susumu Yui, Masanori Honjo
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Abstract
This invention intends to develop many DNA markers for a plant of the genus Fragaria and identify everbearing lines with high precision by using the many DNA markers. The marker associated with everbearing properties in a plant of the genus Fragaria comprises a continuous nucleic acid region sandwiched between the nucleotide sequence as shown in SEQ ID NO: 1 and the nucleotide sequence as shown in SEQ ID NO: 5 in the chromosome of the plant of the genus Fragaria.
Claims4
A method for producing a plant line of the genus Fragaria, comprising:
- (a) extracting genomic DNA from a progeny plant whose at least one parent is a plant of the genus Fragaria or genomic DNA from a plant of the genus Fragaria;
- (b) determining by nucleic acid assay the quantity of a nucleic acid marker associated with everbearing properties in the genomic DNA of the plant, wherein the marker comprises the nucleotide sequence of SEQ ID NO:
- 3;
- (c) detecting a plant with an increased quantity of the marker compared to the quantity detected in one-season-bearing plants;
- (d) selecting the plant as a plant with everbearing properties;
- and
- (e) using the selected plant as a parent plant for crossing to thereby produce a plant line of the genus Fragaria.
Dependencies are inferred from the claim text.