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US 10,704,091 B2Granted
Genotyping by next-generation sequencing
AssigneeData2Bio
InventorsPatrick S. Schnable, Sanzhen Liu, Wei Wu
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Abstract
Provided herein is technology relating to genotyping and particularly, but not exclusively, to methods for genotyping one or more organisms by genome sequencing.
Claims11
A composition comprising:
- a) a nucleic acid template comprising single-stranded ends, said nucleic acid template comprising:
- i) a double-stranded restriction fragment;
- ii) a first single-stranded oligonucleotide ligated to a 3′ end of a first strand of the double-stranded restriction fragment, said first single-stranded oligonucleotide comprising a sequence comprising a first primer binding site;
- and
- ii) a second single-stranded oligonucleotide ligated to a 5′ end of said first strand of the double-stranded restriction fragment, said second single-stranded oligonucleotide comprising a sequence complementary to a second primer binding site;
- b) a first primer capable of hybridizing to said first primer binding site;
- and
- c) a second primer capable of hybridizing to said second primer binding site.
5 more elements
Dependencies are inferred from the claim text.