Methods and kits for theranostic applications
AssigneeTriBiotica LLC
InventorsIan Dunn, Matthew Lawler
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Abstract
The present disclosure is directed to methods and kits for identifying, enriching, and evaluating templated assembly reactants. Some embodiments disclose methods for identifying templated assembly targets by synthesizing templated assembly reactants, hybridizing the templated assembly reactants to target nucleic acids, performing a templated assembly reaction, and identifying the target nucleic acids that hybridized to the templated assembly reactants. Libraries of templated assembly reactants, a kit for identifying templated assembly targets, and a pair of templated assembly targets enriched from a library of chemically-ligated oligonucleotides spatially elicited (CLOSE) products are also disclosed.
Claims10
A method for identifying templated assembly targets comprising:
- synthesizing a first population of templated assembly reactants and a second population of corresponding templated assembly reactants, wherein the first and second populations of templated assembly reactants comprise oligonucleotide sequences;
- hybridizing both populations of templated assembly reactants to target nucleic acids, wherein the target nucleic acids maintain their native secondary structures and wherein the hybridization target sites are discontinuous;
- performing a templated assembly reaction, wherein the hybridized first population of templated assembly reactants and the hybridized second population of corresponding templated assembly reactants undergo templated assembly;
- and
- identifying the target nucleic acids that hybridized to either the first or second population of templated assembly reactants that underwent templated assembly, wherein the hybridized target nucleic acids are the templated assembly targets.
Dependencies are inferred from the claim text.