Method for determining the reaction of a microorganism to its exposure to a chemical compound

Abstract

The invention concerns a method for determining the reaction of at least one bacterium of interest to its exposure to an antibiotic implementing a Raman spectroscopy analysis comprising the following steps: Having a biological sample that could contain said bacteria of interest, Preparing at least two fractions of said sample each comprising one or more living bacteria of interest, Capturing, in each fraction, at least one living bacterium of interest by using a binding partner, Exposing at least one of the fractions to at least one concentration of at least one given antibiotic, the other of the fractions being the control fraction, Submitting the bacterium/bacteria of interest contained in the fractions to an incident light and analyzing the resultant light obtained by Raman diffusion by the bacterium/bacteria of interest by Raman spectroscopy in order to obtain as many Raman spectra as bacteria, Treating said spectra in order to obtain a signature of the reaction of the or each bacterium/bacteria of interest to the exposure to said antibiotic and of the control, Comparing the signature obtained accordingly per bacterium of interest to a reference base defined under the same conditions as above, for different bacteria and at least said antibiotic, and Defining a sensitivity clinical profile of said bacterium of interest to said antibiotic.

Dependencies are inferred from the claim text.