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US 10,704,069 B2Granted
Enzymatic production of D-allulose
AssigneeBonumose, Inc.
InventorsDaniel Joseph Wichelecki, Edwin O. Rogers
Life of this patent
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Abstract
The current disclosure provides a process for enzymatically converting a saccharide into allulose. The invention also relates to a process for preparing allulose where the process involves converting fructose 6-phosphate (F6P) to allulose 6-phosphate (A6P), catalyzed by allulose 6-phosphate 3-epimerase (A6PE), and converting the A6P to allulose, catalyzed by allulose 6-phosphate phosphatase (A6PP).
Claims27
An enzymatic process for preparing D-allulose, the process comprising the steps of:
- (i) converting fructose 6-phosphate (F6P) to allulose 6-phosphate (A6P), catalyzed by an allulose 6-phosphate 3-epimerase comprising the amino acid sequence which has at least 85% sequence identity to SEQ ID NO:3 or 6;
- and (ii) converting the A6P produced to allulose, catalyzed by an allulose 6-phosphate phosphatase comprising the amino acid sequence which has at least 85% sequence identity to SEQ ID NO:9.
An enzymatic process for preparing D-allulose from a saccharide, the process comprising the process steps of:
- (i) converting fructose 6-phosphate (F6P) to allulose 6-phosphate (A6P), catalyzed by an allulose 6-phosphate 3-epimerase (A6PE) comprising the amino acid sequence which has at least 85% sequence identity to SEQ ID NO:3 or 6;
- and (ii) converting the A6P produced to allulose, catalyzed by an allulose 6-phosphate phosphatase (A6PP) comprising the amino acid sequence which has at least 85% sequence identity to SEQ ID NO:9;
- wherein the process steps are conducted at phosphate concentration from 0.1 mM to 150 mM.
Dependencies are inferred from the claim text.