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US 10,704,068 B2Granted
Method of producing heparan sulfate having anticoagulant activity
AssigneeAjinomoto Co., Inc.
InventorYuriko Tokura
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Abstract
The present invention provides a method of producing a heparan sulfate having an anticoagulant activity. Specifically, the present invention provides the method of producing a heparan sulfate having a desired molecular weight, by partially N-deacetylating a heparosan followed by depolymerizing the heparosan by heparinase III, and converting the produced low molecular weight product into heparan sulfate having an anticoagulant activity.
Claims11
A method of producing a heparan sulfate having anticoagulant activity, comprising the following steps:
- (A) partially N-deacetylating a heparosan to produce an N-deacetylated heparosan,
- (B) treating the N-deacetylated heparosan with heparinase Ill to produce a depolymerized N-deacetylated heparosan, and
- (C) sulfating the depolymerized N-deacetylated heparosan to produce the heparan sulfate having anticoagulant activity, wherein said step (A) is performed so that a residual rate of N-acetylated groups is 1% to 33%.
Dependencies are inferred from the claim text.