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US 10,704,066 B2Granted
Neurotransmitters and methods of making the same
AssigneePurissima, Inc.
InventorsGeorge Rudenko, Robert Evans
Life of this patent
Estimating the term…
Abstract
In an aspect, the disclosure provides methods for making neurotransmitters in a host organism. The neurotransmitters can be cannabinoids and derivatives of cannabinoids. The host cells can be microalgae, fungi or other host cells. In a related aspect, the disclosure provides host cells engineered to have biochemical pathways for making neurotransmitters such as cannabinoids.
Claims20
A method, comprising the steps of:
- providing a Prototheca microalgae host cell wherein the Prototheca microalgae host cell comprises a first nucleic acid encoding a hexanoyl-CoA synthase from Cannabis sativa, a second nucleic acid encoding a 3,5,7-trioxododecanoyl-CoA synthase from Cannabis sativa, a third nucleic acid encoding an olivetolic acid cyclase from Cannabis sativa, and a fourth nucleic acid encoding a geranyl-diphosphate:olivetolate geranyltransferase from Cannabis sativa, wherein the first nucleic acid is operably linked to a control region, wherein the second nucleic acid is operably linked to a control region, wherein the third nucleic acid is operably linked to a control region, and wherein the fourth nucleic acid is operably linked to a control region, introducing the microalgae cell into a media wherein the media comprises a carbon source, culturing the microalgae cell in the media whereby a cannabinoid is made.
Dependencies are inferred from the claim text.