Constitutively active ABA receptor mutants
AssigneeThe Regents of the University of California
InventorsSean R. Cutler, Assaf Mosquna
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Abstract
The present invention provides for compositions comprising mutated PYR/PYL receptor polypeptides that bind to a type 2 protein phosphatase in the absence of abscisic acid. The present invention further provides for methods of making and using the mutated PYR/PYL receptor polypeptides.
Claims18
An isolated nucleic acid comprising a polynucleotide encoding a mutated PYR/PYL receptor polypeptide comprising one or more amino acid substitutions in a ligand-binding pocket as compared to a wild-type PYR/PYL receptor polypeptide, wherein the mutated PYR/PYL receptor binds to a type 2 protein phosphatase (PP2C) in the absence of abscisic acid, wherein:
- the mutated PYR/PYL receptor polypeptide comprises amino acid substitutions corresponding to the amino acid substitutions H60P, V83F, and F159V in PYR1 as set forth in SEQ ID NO:1;
- or
- the mutated PYR/PYL receptor polypeptide comprises amino acid substitutions corresponding to the amino acid substitutions H60P, V83F, M158I, and F159V in PYR1 as set forth in SEQ ID NO:1;
- or
- the mutated PYR/PYL receptor polypeptide comprises amino acid substitutions corresponding to the amino acid substitutions V83F, M158I, F159V, and K170W in PYR1 as set forth in SEQ ID NO:1;
- and
- wherein further substitutions if any, correspond to positions of amino acid residues described in Table 1.
Dependencies are inferred from the claim text.