Protein having synthetic activity for imidazole dipeptid and production method of imidazole dipeptide
AssigneeTokai Bussan Co., Ltd.
InventorKuniki Kino
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Abstract
The present invention provides L-amino acid α-ligase represented by sequence number 1 (SEQ ID NO. 1), which is a mutant protein including substitution of at least 1-3 amino acid residues of amino acid sequence of protein YwfE, with the substitution being at least one of an asparagine (N) residue of the 108th place from the N terminal being substituted with at least one of an alanine (A) residue, a glutamic acid (E) residue and a glutamine (Q) residue, an isoleucine (I) residue of the 112th place from the N terminal being substituted with a valine (V) residue, and a histidine (H) residue of the 378th place from the N terminal being substituted with at least one of a lysine (K) residue or an arginine (R) residue.
Claims13
A mutant protein of L-amino acid α-ligase represented by wild type SEQ ID NO. 1, the mutant protein including a substitution of at least 1-3 amino acid residues of amino acid sequence of protein YwfE, with the substitution being at least one of:
- an asparagine (N) residue of a 108th place from an N terminal of the mutant protein being substituted with at least one of an alanine (A) residue, a glutamic acid (E) residue and a glutamine (Q) residue,
- an isoleucine (I) residue of a 112th place from the N terminal of the mutant protein being substituted with a valine (V) residue, and
- a histidine (H) residue of a 378th place from the N terminal of the mutant protein being substituted with at least one of a lysine (K) residue and an arginine (R) residue,
- the mutant protein being a protein having an amino acid sequence selected from the group consisting of:
- SEQ ID NO. 2 (N108A), SEQ ID NO. 3 (N108E), SEQ ID NO. 4 (N108Q), SEQ ID NO. 5 (N108A/I112V), SEQ ID NO. 6 (N108A/H378K), SEQ ID NO. 7 (N108A/H378R), SEQ ID NO. 8 (N108E/I112V), SEQ ID NO. 9 (N108E/H378K), SEQ ID NO. 10 (N108E/H378R), SEQ ID NO. 11 (N108Q/I112V), SEQ ID NO. 12 (N108Q/H378K), SEQ ID NO. 13 (N108Q/H378R), SEQ ID NO. 14 (N108A/I112V/H378R), SEQ ID NO. 15 (N108Q/I112V/H378K) and SEQ ID NO. 16 (N108Q/I112V/H378R) the mutant having L-amino acid α-ligase activity, and the N terminal of the mutant protein being a residue located at the position 1 of the amino acid sequence.
Dependencies are inferred from the claim text.