DNA polymerase mutants with increased processivity of DNA synthesis
AssigneeJiangnan University
InventorsJing Wu, Li Wang
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Abstract
Disclosed are Dpo4 DNA polymerase mutants with increased DNA synthesis processivity. The mutant enzymes, Dpo4 A181D and Dpo4 E63K, are constructed based on the wild-type Dpo4 derived from Sulfolobus solfataricus to obtain Dpo4DNA polymerase mutants with increased DNA processivity. The extension length of Dpo4 A181D and Dpo4 E63K are respectively increased by 25% and 18.8% than that of the wild-type Dpo4. The fidelity of Dpo4 A181D and wild-type Dpo4 are similar, and the fidelity of Dpo4 E63K is increased from that of the wild-type Dpo4. In summary, the mutants Dpo4 A181D and Dpo4 E63K obtained by the present invention have increased processivity compared to that of the wild-type Dpo4 DNA polymerase.
Claims5
A Dpo4 DNA polymerase mutant with increased processivity as compared to its wild-type counterpart enzyme, wherein the wild-type enzyme is Dpo4 DNA polymerase having the amino acid sequence of SEQ ID NO:
- 2, and wherein the Dpo4 DNA polymerase mutant has a single-point mutation with mutating Alanine at the 181st position of the wild-type Dpo4 DNA polymerase to Aspartic acid, or Glutamic acid at the 63rd position to Lysine.
Dependencies are inferred from the claim text.