Mutant 3-hydroxybutyrate dehydrogenase from Rhodobacter sphaeroides as well as methods and uses involving the same
AssigneeRoche Diabetes Care, Inc.
InventorsDaniela Beck, Mara Boenitz-Dulat, Peter Kratzsch, Thomas Streidl, Carina Horn
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Abstract
The present invention relates to a mutant 3-hydroxybutyrate dehydrogenase (3-HBDH) with improved performance relative to the wild-type 3-HBDH, a nucleic acid encoding the mutant 3-HBDH, a cell comprising the mutant 3-HBDH or the nucleic acid, a method of determining the amount or concentration of 3-hydroxybutyrate in a sample, and a device for determining the amount or concentration of 3-hydroxybutyrate in a sample.
Claims20
A mutant 3-hydroxybutyrate dehydrogenase (3-HBDH) from Rhodobacter sphaeroides with improved performance relative to the wild-type 3-HBDH, wherein the mutant comprises an amino acid sequence that is at least 80% identical to the amino acid sequence of SEQ ID NO:
- 1 (wild type 3-HBDH from Rhodobacter sphaeroides) and wherein the mutant has at least one amino acid substitution relative to the wild-type 3-HBDH at a position corresponding to position 250 of SEQ ID NO:
- 1, wherein, the improved performance is at least increased thermal stability relative to the wild-type 3-HBDH.
Dependencies are inferred from the claim text.