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US 10,703,971 B2Granted
Chemiluminescent substrates
AssigneeBeckman Coulter, Inc.
InventorsRenuka De Silva, Robert A. Eickholt, Jaya S. Koti, Mark D. Sandison, David L. Schumm, Wenhua Xie
Life of this patent
Estimating the term…
Abstract
Alkaline phosphatase chemiluminescent substrate formulations are provided exhibiting rapid incubation periods and improved stability for use in immunoassays.
Claims19
A substrate formulation which produces chemiluminescence in the presence of a phosphatase enzyme and which comprises in an aqueous solution:
- a) 0.01 mM-50 mM of a chemiluminescent compound of formula I or a salt thereof:
- wherein
- A is C1-6haloalkyl, naphthyl, phenyl, substituted phenyl, or heteroaryl, wherein substituted phenyl comprises from 1 to 3 halo, C1-6 alkyl, C1-6 alkoxy, C1-6 haloalkyl, C(O)R15, CN or NO2 substituents;
- R1 is selected from the group consisting of C5-14aryl, C1-6 alkyl, C1-6 haloalkyl, and C5-14 aralkyl groups;
- R7-R14 are independently H, C1-6 alkoxy, halo, C1-4alkyl, or R7 or R8-R9 or R9-R10 or R11-R12 or R12-R13 or R13-R14, can be joined together as a carbocyclic or heterocyclic ring system comprising at least one 5 or 6-membered ring;
- R15 is C1-6 alkyl;
- each M is independently selected from the group consisting of H, an alkali metal, alkaline earth metal, transition metal, ammonium, phosphonium, organic amine salt, and an amino acid salt;
- Z is O or S;
- and
- n is 0, 1, or 2;
- b) 0.01-200 μM of a cationic aromatic compound (CAC);
- c) 1 μM-10 mM of a background reducing agent;
- and
- d) 0.05-20 g/L of an ether-linked nonionic surfactant that does not contain a carboxylate ester group;
- or a hydrophilic polymer.
12 more elements
A substrate formulation which produces chemiluminescence in the presence of a phosphatase enzyme, wherein the composition comprises in an aqueous solution:
- a) a chemiluminescent compound of formula (I) or a salt thereof:
- wherein
- A is C1-6haloalkyl, naphthyl, phenyl, substituted phenyl, or heteroaryl, wherein substituted phenyl comprises from 1 to 3 halo, C1-6 alkyl, C1-6 alkoxy, C1-6 haloalkyl, C(O)R15, CN or NO2 substituents;
- R1 is selected from the group consisting of C5-14aryl, C1-6 alkyl, C1-6 haloalkyl, and C5-14 aralkyl groups;
- R7-R14 are independently H, C1-6 alkoxy, halo, or C1-4alkyl, or R7-R8 or R8-R9 or R9-R10 or R11-R12 or R12-R13 or R13-R14, can be joined together as a carbocyclic or heterocyclic ring system comprising at least one 5 or 6-membered ring;
- R15 is C1-6 alkyl;
- M is independently selected from H, Li, Na, or K;
- Z is O or S;
- and
- n is 0, 1, or 2;
- b) a cationic aromatic compound in an amount effective to increase the chemiluminescence compared to that generated in the absence of the cationic aromatic compound;
- and
- c) a background reducing agent,
- wherein the improvement comprises
- d) an ether-linked non-ionic surfactant that does not contain a carboxylate ester group and/or a hydrophilic polymer in the aqueous solution,
- wherein the resultant turnaround time (TAT) is less than 2 minutes following exposure to alkaline phosphatase in an automated immunoassay or Performance Test B.
13 more elements
Dependencies are inferred from the claim text.