Antibody-mediated transduction of heat shock proteins into living cells

A method for inhibiting or treating a subject suffering a disease or disorder comprising administering a suitable amount of a pharmaceutical composition comprising fusion protein comprising a 3E10 Fv joined to a Hsp-70, a peptide linker comprising a portion of an immunoglobulin heavy chain constant domain CH1, and a swivel sequence, and optionally, the 3E10 Fv comprising an amino acid sequence AGIH, as shown in SEQ ID NO:37, at its amino terminus, wherein the swivel sequence of the linker is a peptide sequence that is located between the 3E10 Fv and the Hsp-70 and permits the 3E10 Fv and the Hsp-70 to swivel A method for inhibiting a disease or disorder by promoting hydrogen peroxide or reactive oxygen species (ROS) cytoprotection comprising administering a pharmaceutical composition comprising fusion protein comprising a 3E10 Fv joined to a Hsp-70, a peptide linker comprising a portion of an immunoglobulin heavy chain constant domain CH1, and a swivel sequence, and optionally, the 3E10 Fv comprising an amino acid sequence AGIH, as shown in SEQ ID NO:37, at its amino terminus, wherein the swivel sequence of the linker is a peptide sequence that is located between the 3E10 Fv and the Hsp-70 and permits the 3E10 Fv and the Hsp-70 to swivel, wherein the swivel sequence is a peptide sequence consisting of LESSGS beginning at position 375 and ending at position 380 of SEQ ID NO:

• 2, and pharmaceutically acceptable carriers, binders, diluents, adjuvants, excipients, and/or vehicles, thereby inhibiting the disease or disorder, and pharmaceutically acceptable carriers, binders, diluents, adjuvants, excipients, and/or vehicles to the subject, thereby inhibiting or treating the disease or disorder.