Configurational stereoisomer of bromadiolone, composition and rodenticide bait comprising same, and method for controlling target rodent pests
AssigneesLiphatech, Institut Enseignement Superieur Et Recherche En Alimentation Sante Animale Sciences Agronomiques Et Environnement (Vet Agro Sup)
InventorsHervé Caruel, Etienne Benoit, Isabelle Fourel, Virginie Lattard
Life of this patent
Estimating the term…
Abstract
Disclosed is a configurational stereoisomer, named enantiomer E1, of bromadiolone having, by chromatographic analysis of bromadiolone including four configurational stereoisomers of bromadiolone performed under the conditions described below, a retention time t1 having a value such that t1<t2<t3<t4; t2, t3 and t4 being the retention times of the configurational stereoisomers of bromadiolone different from the enantiomer E1, the analysis being performed at a temperature of 27.3° C.
Claims20
Configurational stereoisomer, named enantiomer E1, of bromadiolone, said enantiomer E1 being in an isolated state and presenting, under chromatographic analysis of a composition comprising four configurational stereoisomers of performed under the conditions described below, a retention time t1 having a value such that t1<t2<t3<t4;
- t2, t3 and t4 being the retention times of the configurational stereoisomers of bromadiolone different from said enantiomer E1, said analysis being performed at a temperature of 27.3° C. and under the following conditions:
- using a high-pressure liquid chromatography column of dimensions 150×2 mm, and containing a chiral stationary phase constituted of particles of cellulose tris(3,5-dimethylphenyl carbamate), said particles having a mean size of 3 μm and having a mean pore size of 1000 Å, and;
- using, as liquid mobile phase, a mixture formed from acetonitrile (A) and water comprising 0.1% by volume of formic acid (B), with an AB volume ratio of 80/20 and with a flow rate of the liquid mobile phase in the chromatography column of 0.25 mL/minute, and;
- by injection into the chromatography column of a volume of 1 μL of bromadiolone composition at a concentration of 1 μg of bromadiolone per millilitre of acetonitrile.
Composition comprising a configurational stereoisomer, named enantiomer E1, of bromadiolone, with the exclusion of a racemic mixture of said enantiomer E1 and of another configurational stereoisomer, named enantiomer E2, of bromadiolone different from said enantiomer E1, said enantiomer E1 and said enantiomer E2 being enantiomers of one diastereoisomer of the two diastereoisomers of bromadiolone;
- said enantiomer E1 having, by chromatographic analysis of a bromadiolone composition comprising four configurational stereoisomers of bromadiolone performed under the conditions described below, a retention time t1;
- said enantiomer E2 having, by chromatographic analysis of a bromadiolone composition comprising four configurational stereoisomers of bromadiolone performed under these same conditions, a retention time t2;
- t1 and t2 being values such that t1<t2<t3<t4;
- t3 and t4 representing the retention times of configurational stereoisomers of bromadiolone different from said enantiomer E1 and from said enantiomer E2, said analysis being performed at a temperature of 27.3° C. and under the following conditions:
- using a high-pressure liquid chromatography column of dimensions 150×2 mm, and containing a chiral stationary phase constituted of particles of cellulose tris(3,5-dimethylphenyl carbamate) particles, said particles having a mean size of 3 μm and having a mean pore size of 1000 Å, and;
- using, as liquid mobile phase, a mixture formed from acetonitrile (A) and water comprising 0.1% by volume of formic acid (B), with an AB volume ratio of 80/20 and with a flow rate of the liquid mobile phase in the chromatography column of 0.25 mL/minute;
- by injection into the chromatography column of a volume of 1 μL of bromadiolone composition at a concentration of 1 μg of bromadiolone per millilitre of acetonitrile.
Process for controlling target rodent pests, in which there is spread an amount of rodenticidal bait comprising:
- at least one excipient that is edible for target rodent pests;
- and
- a configurational stereoisomer, named enantiomer E1, of bromadiolone, with the exclusion of a racemic mixture of said enantiomer E1 and of another configurational stereoisomer, named enantiomer E2, of bromadiolone different from said enantiomer E1 said enantiomer E1 and said enantiomer E2 being enantiomers of one diastereoisomer of the two diastereoisomers of bromadiolone;
- said enantiomer E1 presenting, under chromatographic analysis of a bromadiolone composition comprising four configurational stereoisomers of bromadiolone performed under the conditions described below, a retention time t1;
- said enantiomer E2 having, by chromatographic analysis of a bromadiolone composition comprising four configurational stereoisomers of bromadiolone performed under these same conditions, a retention time t2;
- t1 and t2 being values such that t1<t2<t3<t4;
- t3 and t4 representing the retention times of configurational stereoisomers of bromadiolone different from said enantiomer E1 and from said enantiomer E2, said analysis being performed at a temperature of 27.3° C. and under the following conditions:
- using a high-pressure liquid chromatography column of dimensions 150×2 mm, and containing a chiral stationary phase constituted of particles of cellulose tris(3,5-dimethylphenyl carbamate), said particles having a mean size of 3 μm and having a mean pore size of 1000 Å, and;
- using, as liquid mobile phase, a mixture formed from acetonitrile (A) and water comprising 0.1% by volume of formic acid (B), with an A/B volume ratio of 80/20 and with a flow rate of the liquid mobile phase in the chromatography column of 0.25 mL/minute, and;
- by injection into the chromatography column of a volume of 1 μL of bromadiolone composition at a concentration of 1 μg of bromadiolone per millilitre of acetonitrile.
Dependencies are inferred from the claim text.