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US 10,702,630 B2Granted
Tubular tissue construct and a method of printing
AssigneePresident and Fellows of Harvard College
InventorsJennifer A. Lewis, Kimberly A. Homan, David B. Kolesky, Ryan L. Truby, Mark A. Skylar-Scott
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Abstract
A 3D printed tubular construct, such as a nephron, with or without embedded vasculature as well as methods of printing tubular tissue constructs are described.
Claims24
A method of printing a tubular tissue construct comprising:
- depositing one or more sacrificial filaments on or in a substrate to form a functional channel pattern, each sacrificial filament comprising a fugitive ink and a plurality of predetermined types of viable cells, wherein each predetermined type of viable cells is deposited at a different predetermined location along a length of the sacrificial filament;
- at least partially surrounding the functional channel pattern with an extracellular matrix composition,
- removing the fugitive ink to create one or more functional channels in the extracellular matrix composition, at least a portion of each different predetermined type of viable cells remaining at the different predetermined location after removal of the fugitive ink, thereby forming a tubular tissue construct.
A method of printing a tubular tissue construct comprising:
- depositing one or more sacrificial filaments on or in a substrate to form a functional channel pattern, each sacrificial filament comprising a fugitive ink and a plurality of predetermined types of binding domains, wherein each predetermined type of binding domain is deposited at a different predetermined location along a length of the sacrificial filament and is capable of binding to a predetermined type of target cell;
- at least partially surrounding the functional channel pattern with an extracellular matrix composition,
- removing the fugitive ink to create one or more functional channels in the extracellular matrix composition, at least a portion of the different predetermined types of binding domains remaining at the different predetermined locations after removal of the fugitive ink;
- and
- injecting a suspension comprising at least one predetermined type of target cells into the functional channel, wherein the target cells bind to corresponding predetermined types of binding domains, thereby forming a tubular tissue construct.
2 more elements
A method of printing a tubular tissue construct, the method comprising:
- depositing one or more cell-laden filaments each comprising a plurality of predetermined types of viable cells on or in the substrate to form one or more tissue patterns, each of the tissue patterns comprising at least two predetermined cell types, wherein each predetermined type of viable cells is deposited at a different predetermined location along a length of the cell-laden filament;
- depositing one or more sacrificial filaments on or in the substrate to form a functional channel pattern interpenetrating the one or more tissue patterns, each of the sacrificial filaments comprising a fugitive ink;
- at least partially surrounding the one or more tissue patterns and the functional channel pattern with an extracellular matrix composition,
- removing the fugitive ink to create functional channels in the extracellular matrix composition, thereby forming an interpenetrating channel network in a tissue construct.
1 more element
Dependencies are inferred from the claim text.