Use of hydrophobic organic acids to increase hydrophobicity of proteins and protein conjugates
AssigneeRezolute, Inc.
InventorsMary S. Rosendahl, Sankaram B. Mantripragada, Eliana B. Gomez
Life of this patent
Estimating the term…
Abstract
Examples may include a method of making a protein-PEG conjugate salt with increased hydrophobicity. The method may include providing an aqueous protein solution. This aqueous protein solution may include a protein and a pH buffer. The method may also include reacting a polyethylene glycol with the protein to form a protein-PEG conjugate. The method may further include protonating an amino group on the protein-PEG conjugate with a hydrophobic organic acid in an organic phase. The protonation may form the protein-PEG conjugate salt having a hydrophobic anion that increases the hydrophobicity-PEG conjugate salt.
Claims17
A method of making a protein-PEG conjugate salt with increased hydrophobicity, the method comprising:
- providing an aqueous protein solution comprising a protein and a pH buffer;
- reacting a polyethylene glycol with the protein to form a protein-PEG conjugate;
- and
- protonating an amino group on the protein-PEG conjugate with an organic acid, wherein:
- the protonation forms the protein-PEG conjugate salt having a hydrophobic anion that increases the hydrophobicity of the protein-PEG conjugate salt,
- protonating the amino group on the protein-PEG conjugate with the organic acid is in a water-immiscible solvent in an organic phase without the presence of water, and
- the organic acid comprises pamoic acid, docusate hydrogen, furoic acid, or mixtures thereof.
A method of making a protein salt with increased hydrophobicity, the method comprising:
- providing an aqueous protein solution comprising a protein and a pH buffer;
- and
- protonating an amino group on the protein with an organic acid, wherein:
- the protonation forms the protein salt having a hydrophobic anion that increases the hydrophobicity of the protein salt,
- protonating the amino group on the protein with the organic acid is in a water-immiscible solvent in an organic phase without the presence of water, and
- the organic acid comprises pamoic acid, docusate hydrogen, furoic acid, or mixtures thereof.
Dependencies are inferred from the claim text.