Methods and compositions for inactivating enveloped viruses
AssigneeBiogen MA Inc.
InventorsLynn Edward Conley, Yinying Tao
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Abstract
Aspects of the invention provide therapeutic recombinant protein preparations and methods of preparing therapeutic recombinant protein, which methods include contacting recombinant protein preparations with zwitterionic detergent at or above critical micelle concentration of the detergent.
Claims15
A method of preparing a recombinant protein, the method comprising contacting a recombinant protein preparation with a zwitterionic detergent that is N,N-dimethyldodecylamine N-oxide (LDAO), N-dodecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate (sulfobetaine 3-12), N-tetradecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate (sulfobetaine 3-14), or N-hexadecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate (sulfobetaine 3-16) in an amount sufficient to inactivate an enveloped virus that contaminates the recombinant protein preparation, wherein the amount is at a concentration above critical micelle concentration of the detergent and does not inhibit biological activity of the recombinant protein.
Dependencies are inferred from the claim text.