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US 10,702,588 B2Granted
Modified relaxin polypeptides comprising a non-naturally encoded amino acid in the A chain
AssigneeAmbrx, Inc.
InventorsVadim Kraynov, Nick Knudsen, Amha Hewet, Kristine De Dios, Jason Pinkstaff, Lorraine Sullivan
Life of this patent
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Abstract
Modified relaxin polypeptides and their uses thereof are provided.
Claims20
A modified relaxin polypeptide comprising a non-naturally encoded amino acid, wherein:
- (a) the relaxin polypeptide comprises a relaxin A chain polypeptide and a relaxin B chain polypeptide, wherein said relaxin A chain polypeptide has a sequence at least 95% identical to SEQ ID NO:
- 4, and said relaxin B chain polypeptide has a sequence at least 95% identical to SEQ ID NO:
- 5 or SEQ ID NO:
- 6, and said non-naturally encoded amino acid is substituted in said A chain polypeptide at residue 1;
- and (b) said non-naturally encoded amino acid is linked to a linker or polymer, wherein said non-naturally encoded amino acid comprises a first functional group and the linker or polymer comprises a second functional group, wherein the first functional group and second functional group are not identical and each comprise a carbonyl group, an aminooxy group, a hydrazide group, a hydrazine group, a semicarbazide group, an azide group, or an alkyne group, and the resultant covalent linkage created by the reaction of the first and second functional groups comprises a triazole or an oxime linkage.
2 more elements
A modified relaxin polypeptide comprising a non-naturally encoded amino acid, wherein:
- (a) the relaxin polypeptide comprises a relaxin A chain polypeptide and a relaxin B chain polypeptide, wherein said relaxin A chain polypeptide has a sequence at least 95% identical to SEQ ID NO:
- 4, and said relaxin B chain polypeptide has a sequence at least 95% identical to SEQ ID NO:
- 5 or SEQ ID NO:
- 6, and said non-naturally encoded amino acid is substituted in said A chain polypeptide at residue 1;
- and (b) said non-naturally encoded amino acid is linked to a linker or polymer by a triazole or an oxime linkage.
2 more elements
A modified relaxin polypeptide comprising a non-naturally encoded amino acid, wherein:
- (a) the relaxin polypeptide comprises a relaxin A chain polypeptide and a relaxin B chain polypeptide, wherein said relaxin A chain polypeptide comprises the amino acid sequence of SEQ ID NO:
- 4 substituted with said non-naturally encoded amino acid at residue 1, and said relaxin B chain polypeptide comprises the amino acid sequence of SEQ ID NO:
- 5 or SEQ ID NO:
- 6;
- and (b) said non-naturally encoded amino acid is linked to a linker or polymer by a triazole or an oxime linkage.
2 more elements
Dependencies are inferred from the claim text.