Methods and compounds to suppress viral genome release and packaging
AssigneeCarnegie Mellon University
InventorAlex Evilevitch
Life of this patent
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Abstract
Provided herein are methods of suppressing viral nucleic acid, e.g. double-stranded (ds) DNA, genome release from or packaging of viruses having their nucleic acid genome packaged under stress in their capsid, and compositions useful for that purpose. The methods alter the ionic environment of the nucleic acid within the capsid and thereby prevent release of, and/or interfere with packaging of the viral genome.
Claims15
A method of treating an infection in a patient of a herpesvirus having a stressed nucleic acid genome in a capsid, comprising administering to a patient in need thereof a composition comprising a polypeptide, the polypeptide having an amino acid sequence consisting of a sequence selected from the group consisting of Lys3+, Lys4+ (SEQ ID NO:
- 9), Lys5+ (SEQ ID NO:
- 10), Lys6+ (SEQ ID NO:
- 11), Lys7+ (SEQ ID NO:
- 25), Lys8+ (SEQ ID NO:
- 26), Lys9+ (SEQ ID NO:
- 12), Lys3Arg3 (SEQ ID NO:
- 13), (LysArg)3 (SEQ ID NO:
- 14), Orn3+, Orn4+ (SEQ ID NO:
- 20), and Orn5+ (SEQ ID NO:
- 27) in an amount effective to condense nucleic acid in the capsid of a herpesvirus particle, thereby treating the herpesvirus infection.
A method of treating an infection in a patient of a virus having a stressed nucleic acid genome in a capsid, comprising administering to a patient in need thereof a composition comprising a polycationic dendrimer having an overall positive charge of 3+ or greater at a physiologic pH and a molecular weight of 4000 g/mol or less, in an amount effective to condense nucleic acid in the capsid of the virus particle, thereby treating the virus infection, wherein the dendrimer is G0-PAMAM, DAB-Am-4, and/or DAB-Am-8.
Dependencies are inferred from the claim text.