Catalase inhibitor and method for measuring analyte using catalase inhibitor
AssigneePhc Holdings Corporation
InventorsKeigo Haneda, Takashi Endoh
Life of this patent
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Abstract
Provided is a catalase inhibitor comprising a compound represented by formula (I): wherein R1 to R4 independently represent a hydrogen atom, a halogen atom, an amino group, a hydroxyl group, a carbonyl group, or a hydrocarbon group having 1 to 4 carbon atoms, wherein the hydrocarbon group may have at least one substituent selected from the group consisting of a halogen atom, an amino group, a hydroxyl group and a carbonyl group; and X− represents an anionic chemical species.
Claims12
A method for measuring an analyte based on production of hydrogen peroxide, comprising the following steps:
- (A) a step of mixing a sample and a catalase inhibitor comprising a compound represented by formula (I):
- wherein R1, R2, R3 and R4 each independently represents a hydrogen atom, a halogen, an amino group, a hydroxyl group, a carboxyl group, or a hydrocarbon group having 1 to 4 carbons which may contain substituent(s);
- and X− represents an anionic chemical species, wherein the sample comprises the analyte and a catalase;
- (B) a step of oxidizing the analyte by a first enzyme to produce hydrogen peroxide;
- and
- (C) a step of measuring the hydrogen peroxide.
A method for measuring an analyte based on production of a hydrogen peroxide, comprising the following steps:
- (a) a step of mixing a sample and a catalase inhibitor comprising a compound represented by formula (I):
- wherein R1, R2, R3 and R4 each independently represents a hydrogen atom, a halogen, an amino group, a hydroxyl group, a carboxyl group, or a hydrocarbon group having 1 to 4 carbons which may contain substituent(s);
- and X− represents an anionic chemical species, wherein the sample comprises the analyte and a catalase;
- (b-1) a step of producing a substrate from the analyte by a second enzyme;
- (b-2) a step of oxidizing the substrate by a first enzyme to produce hydrogen peroxide;
- and
- (c) a step of measuring the hydrogen peroxide.
A measurement kit which measures an analyte in a sample comprising a catalase, comprising:
- a first enzyme which catalyzes a reaction where the analyte is oxidized to produce hydrogen peroxide;
- and
- a catalase inhibitor comprising a compound represented by formula (I):
- wherein R1, R2, R3 and R4 each independently represents a hydrogen atom, a halogen, an amino group, a hydroxyl group, a carboxyl group, or a hydrocarbon group having 1 to 4 carbons which may contain substituent(s);
- and X− represents an anionic chemical species.
A measurement kit that measures an analyte in a sample comprising a catalase, comprising:
- a first enzyme which catalyzes a reaction where a substrate is oxidized to produce hydrogen peroxide;
- a catalase inhibitor comprising a compound represented by formula (I):
- wherein R1, R2, R3 and R4 each independently represents a hydrogen atom, a halogen, an amino group, a hydroxyl group, a carboxyl group, or a hydrocarbon group having 1 to 4 carbons which may contain substituent(s);
- and X− represents an anionic chemical species;
- and
- a second enzyme which catalyzes a reaction where the substrate is produced from the analyte.
A kit for pretreatment of a sample for measurement of an analyte in the sample comprising a catalase by an electrochemical method, based on production of hydrogen peroxide by oxidization of a substrate by a first enzyme, wherein the kit for pretreatment of the sample comprises:
- a catalase inhibitor comprising a compound represented by formula (I):
- wherein R1, R2, R3 and R4 each independently represents a hydrogen atom, a halogen, an amino group, a hydroxyl group, a carboxyl group, or a hydrocarbon group having 1 to 4 carbons which may contain substituent(s);
- and X− represents an anionic chemical species;
- and
- a second enzyme which catalyzes a reaction where the substrate is produced from the analyte.
Dependencies are inferred from the claim text.