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US 10,697,008 B2Granted
Sample preparation methods, systems and compositions
AssigneeKarius, Inc.
InventorsTimothy A. Blauwkamp, Rene Sit, Igor D. Vilfan
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Abstract
The disclosure provides methods, compositions, systems, and kits for the concurrent detection and analysis of different structural and chemical forms of nucleic acids in a sample.
Claims22
A method for performing a primer extension reaction on RNA and DNA, comprising:
- a) providing a sample comprising a mixture of single-stranded DNA and single-stranded RNA;
- b) attaching a first adapter to said single-stranded DNA,
- c) attaching a second adapter to said single-stranded RNA;
- d) annealing a first primer to said first adapter and annealing a second primer to said second adapter;
- e) extending said annealed first primer on said single-stranded DNA to form double-stranded DNA;
- and
- f) extending said annealed second primer on said single-stranded RNA to form a double-stranded DNA-RNA hybrid.
4 more elements
Dependencies are inferred from the claim text.