Enzymatic production of glycosylated synthons

A process for manufacturing a glycosylated synthon, or monomer, comprising at least one step of placing at least one glycan-saccharase in contact with at least one hydroxylated synthon and at least one sucrose, in which:

• (A) said hydroxylated synthon is chosen from the group constituted of:
• (i) (meth)acrylate/(meth)acrylamide synthons of formula (I):
• in which:
• R1 represents a hydrogen atom or a C1-C3 alkyl;
• R2 represents a C1-C20 alkylene group;
• or a group (C2H4O)n, with n being an integer between 1 and 10;
• and
• X1 represents —(O)—, —(NH)—, —(S)— or —(NR′2(OH))—, with R′2 representing a C1-C20 alkylene group;
• or a group —(C2H4O)m—, with m being an integer between 1 and 10;
• (ii) styrene-based synthons of formula (II):
• in which R3 represents a covalent bond;
• a C1-C20 alkylene group;
• or a group (C2H4O)n, with n being an integer between 1 and 10;
• (iii) N-carboxyanhydride (NCA) synthons of formula (III):
• in which R4 represents a covalent bond;
• a C1-C20 alkylene group;
• or a group (C2H4O)n, with n being an integer between 1 and 10;
• (iv) lactone/lactam/thiolactone synthons of formula (IV):
• in which:
• R5 represents a covalent bond;
• a C1-C20 alkylene group;
• or a group (C2H4O)m, with m being an integer between 1 and 10;
• n represents an integer between 1 and 20;
• X2 represents —(O)—, —(NH)— or —(S)—;
• and
• R6 represents a hydrogen or a C1-C20 alkyl group;
• (v) synthons of formula (V):
• in which R7 represents a C1-C20 alkylene group;
• and
• (vi) synthons of formula (VI):
• in which R8 represents a C1-C20 alkylene group;
• and
• (B) said glycan-saccharase is selected from the group consisting of
• a sequence having at least 80% identity with SEQ ID NO:
• 1,
• a sequence having at least 80% identity with SEQ ID NO:
• 1 mutated once at any one of the positions R226, I228, F229, A289, F290, I330, V331, D394 and R446;
• a sequence having at least 80% identity with SEQ ID NO:
• 11;
• a sequence having at least 80% identity with SEQ ID NO:
• 12;
• a sequence having at least 80% identity with SEQ ID NO:
• 13;
• a sequence having at least 80% identity with SEQ ID NO:
• 14;
• a sequence having at least 80% identity with SEQ ID NO:
• 15;
• a sequence having at least 80% identity with SEQ ID NO:
• 16;
• a sequence having at least 80% identity with SEQ ID NO:
• 17;
• and
• a sequence having at least 80% identity with SEQ ID NO:
• 18.