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US 10,696,984 B2Granted
Packaging cell for making a pseudotyped lentivirus
AssigneeCalifornia Institute of Technology
InventorsPin Wang, Lili Yang, David Baltimore
Life of this patent
Estimating the term…
Abstract
Methods and compositions are provided for delivery of a polynucleotide encoding a gene of interest, typically an antigen, to a dendritic cell (DC). The virus envelope comprises a DC-SIGN specific targeting molecule. The methods and related compositions can be used to treat patients suffering from a wide range of conditions, including infection, such as HIV/AIDS, and various types of cancers.
Claims8
An isolated packaging cell comprising:
- (a) a replication defective lentiviral vector comprising an exogenous polynucleotide of interest;
- (b) a vector encoding a modified alphavirus E2 glycoprotein that specifically binds DC-SIGN;
- and (c) at least one plasmid encoding proteins required for lentiviral particle packaging, wherein the packaging cell is capable of producing a replication defective lentivirus pseudotyped with a virus envelope comprising the modified alphavirus E2 glycoprotein, and wherein the pseudotyped replication deficient lentivirus has increased efficiency in transducing dendritic cells expressing DC-SIGN relative to cell types not expressing DC-SIGN.
Dependencies are inferred from the claim text.