Methods for treating a brain tissue damage by cultured adult pluripotent olfactory stem cells

A method for treating a brain tissue damage, the method comprising intracerebrally administering a composition containing a plurality of cultured adult pluripotent olfactory stem cells (APOSCs) to a subject in need for a treatment of the brain tissue damage, wherein the brain tissue damage results from a cerebral ischemic disease, and wherein the cultured APOSCs express Bmi-1 (B-lymphoma moloney murine leukemia virus insertion region-1, Oct-4 (Octamer-binding transcription factor 4), Sox-2 (Sex-determining region Y (SRY)-box 2), Nanog, SSEA-4 (Stage-specific embryonic antigen-4), ki67, c-Myc, KLF-4 (Kruppel Like Factor 4), K14 (Cytokeratin 14), and ICAM-1 (Intercellular Adhesion Molecule 1) and are obtained by a process comprising:

• i. isolating APOSCs, comprising:
• (a) obtaining an olfactory mucosa tissue of a mammal,
• (b) culturing the olfactory mucosa tissue obtained from step (a) in a medium containing Dulbecco's Modified Eagle Medium/F12 (DMEM/F12 medium), heparin, bFGF, EGF and an antibiotic for 5-7 days to allow for migration of the cells from the cultured tissue,
• (c) isolating adherent cells from step (b);
• ii. culturing the isolated APOSCs in a sphere culture medium comprising DMEM/F12 medium, B27 supplement, bFGF, EGF and an antibiotic;
• and
• iii. collecting the cultured APOSCs that express Bmi-1, Oct-4, Sox-2, Nanog, SSEA-4, ki67, c-Myc, KLF-4, K14 and ICAM-1.