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US 10,696,706 B2Granted
Methods of preparing steviol glycosides and uses of the same
AssigneePureCircle USA Inc.
InventorsAvetik Markosyan, Siew Yin Chow, Khairul Nizam Bin Nawi
Life of this patent
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Abstract
Methods of preparing steviol glycosides, including Rebaudioside D, Rebaudioside E, Rebaudioside M, Rebaudioside N and Rebaudioside O are provided herein. Sweetener and sweetened consumables containing Rebaudioside D, Rebaudioside E, Rebaudioside M, Rebaudioside N and Rebaudioside O are also provided herein.
Claims11
A method for preparing a steviol glycosides composition consisting essentially of the steps of:
- (a) passing an initial mixture of steviol glycosides further comprising a solvent through a column packed with a polymer resin wherein the steviol glycosides are retained weakly in the column;
- and (b) using the solvent to collect a fraction with a high content of Reb D, Reb E, Reb M, Reb N and Reb O to provide a solution with high content of Reb D, Reb E, Reb M, Reb N and Reb O, wherein the Reb DEMNO/T13 SG ratio in the solution is higher than the Reb DEMNO/T13 SG ratio of the initial mixture of steviol glycosides.
Dependencies are inferred from the claim text.